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(A) Representative H&E-stained sections of mouse liver tissues with different treatments. (B) Levels of serum ALT and AST in different groups. (C) mRNA levels of Bcl2 and Bax in mouse liver tissue detected by RT-qPCR. (D, E) Protein levels of BCL-2, BAX, C-caspase 3, and C-PARP in mouse liver tissue detected by Western blotting. ns p > 0.05, * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001. ConA, concanavalin A; MSA, mesaconate; H&E, hematoxylin and eosin; ALT, alanine aminotransferase; AST, aspartate aminotransferase; BCL2, B-cell lymphoma-2; BAX, Bcl2-associated X protein; C-caspase 3, cleaved-caspase 3; C-PARP, cleaved-poly <t>ADP</t> ribose <t>polymerase;</t> GAPDH, glyceraldehyde 3-phosphate dehydrogenase.
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(A) Representative H&E-stained sections of mouse liver tissues with different treatments. (B) Levels of serum ALT and AST in different groups. (C) mRNA levels of Bcl2 and Bax in mouse liver tissue detected by RT-qPCR. (D, E) Protein levels of BCL-2, BAX, C-caspase 3, and C-PARP in mouse liver tissue detected by Western blotting. ns p > 0.05, * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001. ConA, concanavalin A; MSA, mesaconate; H&E, hematoxylin and eosin; ALT, alanine aminotransferase; AST, aspartate aminotransferase; BCL2, B-cell lymphoma-2; BAX, Bcl2-associated X protein; C-caspase 3, cleaved-caspase 3; C-PARP, cleaved-poly <t>ADP</t> ribose <t>polymerase;</t> GAPDH, glyceraldehyde 3-phosphate dehydrogenase.
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(A) Representative H&E-stained sections of mouse liver tissues with different treatments. (B) Levels of serum ALT and AST in different groups. (C) mRNA levels of Bcl2 and Bax in mouse liver tissue detected by RT-qPCR. (D, E) Protein levels of BCL-2, BAX, C-caspase 3, and C-PARP in mouse liver tissue detected by Western blotting. ns p > 0.05, * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001. ConA, concanavalin A; MSA, mesaconate; H&E, hematoxylin and eosin; ALT, alanine aminotransferase; AST, aspartate aminotransferase; BCL2, B-cell lymphoma-2; BAX, Bcl2-associated X protein; C-caspase 3, cleaved-caspase 3; C-PARP, cleaved-poly <t>ADP</t> ribose <t>polymerase;</t> GAPDH, glyceraldehyde 3-phosphate dehydrogenase.
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(A) Representative H&E-stained sections of mouse liver tissues with different treatments. (B) Levels of serum ALT and AST in different groups. (C) mRNA levels of Bcl2 and Bax in mouse liver tissue detected by RT-qPCR. (D, E) Protein levels of BCL-2, BAX, C-caspase 3, and C-PARP in mouse liver tissue detected by Western blotting. ns p > 0.05, * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001. ConA, concanavalin A; MSA, mesaconate; H&E, hematoxylin and eosin; ALT, alanine aminotransferase; AST, aspartate aminotransferase; BCL2, B-cell lymphoma-2; BAX, Bcl2-associated X protein; C-caspase 3, cleaved-caspase 3; C-PARP, cleaved-poly ADP ribose polymerase; GAPDH, glyceraldehyde 3-phosphate dehydrogenase.

Journal: Journal of Clinical and Translational Hepatology

Article Title: Protective Effect of Mesaconate on Autoimmune Hepatitis via Suppression of Inflammatory Response and Oxidative Stress

doi: 10.14218/JCTH.2025.00112

Figure Lengend Snippet: (A) Representative H&E-stained sections of mouse liver tissues with different treatments. (B) Levels of serum ALT and AST in different groups. (C) mRNA levels of Bcl2 and Bax in mouse liver tissue detected by RT-qPCR. (D, E) Protein levels of BCL-2, BAX, C-caspase 3, and C-PARP in mouse liver tissue detected by Western blotting. ns p > 0.05, * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001. ConA, concanavalin A; MSA, mesaconate; H&E, hematoxylin and eosin; ALT, alanine aminotransferase; AST, aspartate aminotransferase; BCL2, B-cell lymphoma-2; BAX, Bcl2-associated X protein; C-caspase 3, cleaved-caspase 3; C-PARP, cleaved-poly ADP ribose polymerase; GAPDH, glyceraldehyde 3-phosphate dehydrogenase.

Article Snippet: Antibodies used included: anti-GAPDH (AB0037, Abways), anti-IL-1β (26048-4-AP, Proteintech), anti-apoptotic B-cell lymphoma 2 (26593-1-AP, Proteintech), anti-Bcl2-associated X protein (50599-2-Ig, Proteintech), anti-cleaved-caspase-3 (#9661, Cell Signaling Technology (CST)), anti-cleaved-poly ADP ribose polymerase (#5625, CST), anti-inducible nitric oxide synthase (18985-1-AP, Proteintech), anti-Janus kinase 1 (JAK1) (66466-1-Ig, Proteintech), anti-phosphorylated JAK1 (phos-JAK1) (#74129, CST), anti-JAK2 (#3230, CST), anti-phosphorylated JAK2 (phos-JAK2) (#3771, CST), anti-signal transducer and activator of transcription 1 (STAT1) (10144-2-AP, Proteintech), and anti-phosphorylated STAT1 (phos-STAT1) (28977-1-AP, Proteintech).

Techniques: Staining, Quantitative RT-PCR, Western Blot